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KMID : 0613820110210040610
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Journal of Life Science
2011 Volume.21 No. 4 p.610 ~ p.612
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Spectrophotometric Assay for Determination of Chlorogenic Acid Using Green Pigment Formation and Quantitative Analysis of Chlorogenic Acid in Blueberry Leaf
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Chung Dong-min
Chung Young-Chul
Chun Hyo-Kon
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Abstract
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We developed a spectrophotometric assay for the quantitative determination of chlorogenic acid based on the formation of green pigment at 50℃ under glycine and alkaline conditions in 96-well plates. The formation of green pigment was linear with a series of chlorogenic acid concentration (0-300 μM). Using this method, the content of chlorogenic acid (12.42 ㎎/g dry weight) in the leaves of blueberry was quantified. This method is high-throughput, cost-effective, rapid, and easy to perform.
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KEYWORD
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Blueberry leaf, chlorogenic acid, green pigment, amino acid, 96 well plate
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